发信人: vonhabsburg (HRH The Duke of Aled KG GCB OM GCVO FRS), 信区: S_Chemistry
标 题: 化院又一篇JACS
发信站: 南京大学小百合站 (Tue Aug 27 01:35:08 2013)
Abstract
This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN)
to realize in situ “off-on” imaging of intracellular telomerase activity.
In the wrapping DNA (O1) sealed MSN probe, a black hole fluorescence quencher
is covalently immobilized on the inner walls of the mesopores, while
fluorescein is loaded in the mesopores. In the presence of telomerase and
dNTPs, the designed O1 can be extended and then leave away from MSN surface
via forming a rigid hairpin-like DNA structure. Thus the O1 can act as a “bio
-gate” to block and release fluorescein for “off-on” switchable fluorescent
imaging. The MSN probe exhibits good performance for sensitive in situ
tracking of telomerase activity in living cells. The practicality of this
protocol has been verified by monitoring the change of cellular telomerase
activity in response to telomerase-related drugs.
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※ 来源:．南京大学小百合站 http://bbs.nju.edu.cn [FROM: 129.67.118.105]
标 题: 化院又一篇JACS
发信站: 南京大学小百合站 (Tue Aug 27 01:35:08 2013)
Abstract
This work designs a telomerase-responsive mesoporous silica nanoparticle (MSN)
to realize in situ “off-on” imaging of intracellular telomerase activity.
In the wrapping DNA (O1) sealed MSN probe, a black hole fluorescence quencher
is covalently immobilized on the inner walls of the mesopores, while
fluorescein is loaded in the mesopores. In the presence of telomerase and
dNTPs, the designed O1 can be extended and then leave away from MSN surface
via forming a rigid hairpin-like DNA structure. Thus the O1 can act as a “bio
-gate” to block and release fluorescein for “off-on” switchable fluorescent
imaging. The MSN probe exhibits good performance for sensitive in situ
tracking of telomerase activity in living cells. The practicality of this
protocol has been verified by monitoring the change of cellular telomerase
activity in response to telomerase-related drugs.
--
※ 来源:．南京大学小百合站 http://bbs.nju.edu.cn [FROM: 129.67.118.105]
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