发信人: GATTACA (gattaca), 信区: S_LifeScience
标 题: 田大成教授实验室的PNAS文章今天正式online
发信站: 南京大学小百合站 (Tue Oct 22 08:59:08 2013)
田大成教授实验室的PNAS文章今天正式online
恭喜田老师和gattaca实验室！
文章链接如下
http://www.pnas.org/content/early/2013/10/16/1318211110.abstract
Rapidly evolving R genes in diverse grass species confer resistance to rice
blast disease
[b]Abstract[/b]
We show that the genomes of maize, sorghum, and brachypodium contain genes
that, when transformed into rice, confer resistance to rice blast disease. The
genes are resistance genes (R genes) that encode proteins with nucleotide-
binding site (NBS) and leucine-rich repeat (LRR) domains (NBS–LRR proteins).
By using criteria associated with rapid molecular evolution, we identified
three rapidly evolving R-gene families in these species as well as in rice,
and transformed a randomly chosen subset of these genes into rice strains
known to be sensitive to rice blast disease caused by the fungus Magnaporthe
oryzae. The transformed strains were then tested for sensitivity or resistance
to 12 diverse strains of M. oryzae. A total of 15 functional blast R genes
were identified among 60 NBS–LRR genes cloned from maize, sorghum, and
brachypodium; and 13 blast R genes were obtained from 20 NBS–LRR paralogs in
rice. These results show that abundant blast R genes occur not only within
species but also among species, and that the R genes in the same rapidly
evolving gene family can exhibit an effector response that confers resistance
to rapidly evolving fungal pathogens. Neither conventional evolutionary
conservation nor conventional evolutionary convergence supplies a satisfactory
explanation of our findings. We suggest a unique mechanism termed “
constrained divergence,” in which R genes and pathogen effectors can follow
only limited evolutionary pathways to increase fitness. Our results open
avenues for R-gene identification that will help to elucidate R-gene vs.
effector mechanisms and may yield new sources of durable pathogen resistance.
--
※ 来源:．南京大学小百合站 http://bbs.nju.edu.cn [FROM: 114.212.234.253]
标 题: 田大成教授实验室的PNAS文章今天正式online
发信站: 南京大学小百合站 (Tue Oct 22 08:59:08 2013)
田大成教授实验室的PNAS文章今天正式online
恭喜田老师和gattaca实验室！
文章链接如下
http://www.pnas.org/content/early/2013/10/16/1318211110.abstract
Rapidly evolving R genes in diverse grass species confer resistance to rice
blast disease
[b]Abstract[/b]
We show that the genomes of maize, sorghum, and brachypodium contain genes
that, when transformed into rice, confer resistance to rice blast disease. The
genes are resistance genes (R genes) that encode proteins with nucleotide-
binding site (NBS) and leucine-rich repeat (LRR) domains (NBS–LRR proteins).
By using criteria associated with rapid molecular evolution, we identified
three rapidly evolving R-gene families in these species as well as in rice,
and transformed a randomly chosen subset of these genes into rice strains
known to be sensitive to rice blast disease caused by the fungus Magnaporthe
oryzae. The transformed strains were then tested for sensitivity or resistance
to 12 diverse strains of M. oryzae. A total of 15 functional blast R genes
were identified among 60 NBS–LRR genes cloned from maize, sorghum, and
brachypodium; and 13 blast R genes were obtained from 20 NBS–LRR paralogs in
rice. These results show that abundant blast R genes occur not only within
species but also among species, and that the R genes in the same rapidly
evolving gene family can exhibit an effector response that confers resistance
to rapidly evolving fungal pathogens. Neither conventional evolutionary
conservation nor conventional evolutionary convergence supplies a satisfactory
explanation of our findings. We suggest a unique mechanism termed “
constrained divergence,” in which R genes and pathogen effectors can follow
only limited evolutionary pathways to increase fitness. Our results open
avenues for R-gene identification that will help to elucidate R-gene vs.
effector mechanisms and may yield new sources of durable pathogen resistance.
--
※ 来源:．南京大学小百合站 http://bbs.nju.edu.cn [FROM: 114.212.234.253]
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